Viral spliced RNA are produced, encapsidated and reverse transcribed during in Vivo woodchuck hepatitis virus infection
Identifieur interne : 000375 ( France/Analysis ); précédent : 000374; suivant : 000376Viral spliced RNA are produced, encapsidated and reverse transcribed during in Vivo woodchuck hepatitis virus infection
Auteurs : Olivier Hantz [France] ; Isabelle Baginski [France] ; Isabelle Fourel [France] ; Isabelle Chemin [France] ; Christian Trepo [France]Source :
- Virology [ 0042-6822 ] ; 1992.
English descriptors
- Teeft :
- Academic press, Acceptor, Acceptor site, Amplification, Amplification primers, Cauliflower mosaic virus, Cdna, Cdna synthesis, Cetus corp, Consensus sequence, Core gene, Core particles, Duck hepatitis, Final volume, Fusion protein, Gene product, Genetic analysis, Genome, Ground squirrel hepatitis virus, Hantz, Hepatitis, Human hepatitis, Human liver tissues, Hybridization, Important role, Initiation codon, Lesser extent, Life cycle, Liver samples, Negative control, Noninfected woodchuck, Northern blot analysis, Nucleotide sequence, Oligonucleotide, Oligonucleotide probe, Oligonucleotide probes, Open reading frames, Poly, Polymerase, Polymerase chain reaction, Positive control, Potential translation product, Pregenomic, Primer, Primer pair, Probe, Rna, Schaller, Sequence analysis, Southern blot analysis, Spacer region, Splice, Splice donor site, Splice junction, Stringent conditions, Surface antigen, Suzuki, Terminal protein, Terminal protein domain, Transcript, Transcriptase, Transcription, Transcription step, Viral, Viral particles, Woodchuck, Woodchuck hepatitis virus, Woodchuck liver, Woodchuck liver tissues.
Abstract
Abstract: By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and partially sequenced. The splicing donor and acceptor sites of this novel RNA are located, respectively, 130 nucleotides downstream of the ATG initiation codon of the core gene and 21 nucleotides upstream of the initiation codon of the pre-S2 surface gene. The splicing event generates a new core-polymerase fusion protein and removes the terminal protein domain and the spacer region of the polymerase gene. A nucleotide probe specific for the splice junction was used following RT-PCR, to further confirm the existence of this spliced RNA in the liver of seven WHV-infected woodchucks. Deleted viral DNA molecules corresponding to the 2.4 kb spliced RNA were also detected in the liver and, to a lesser extent, in the serum of infected woodchucks, suggesting that this spliced RNA can be encapsidated and reverse-transcribed during the course of natural WHV infection.
Url:
DOI: 10.1016/0042-6822(92)91205-9
Affiliations:
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I" first="Isabelle" last="Fourel">Isabelle Fourel</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03</wicri:regionArea><wicri:noRegion>Cedex 03</wicri:noRegion><wicri:noRegion>Cedex 03</wicri:noRegion></affiliation></author><author><name sortKey="Chemin, Isabelle" sort="Chemin, Isabelle" uniqKey="Chemin I" first="Isabelle" last="Chemin">Isabelle Chemin</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03</wicri:regionArea><wicri:noRegion>Cedex 03</wicri:noRegion><wicri:noRegion>Cedex 03</wicri:noRegion></affiliation></author><author><name sortKey="Trepo, Christian" sort="Trepo, Christian" uniqKey="Trepo C" first="Christian" last="Trepo">Christian Trepo</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03</wicri:regionArea><wicri:noRegion>Cedex 03</wicri:noRegion><wicri:noRegion>Cedex 03</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992">1992</date><biblScope unit="volume">190</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="193">193</biblScope><biblScope unit="page" to="200">200</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Acceptor</term><term>Acceptor site</term><term>Amplification</term><term>Amplification primers</term><term>Cauliflower mosaic virus</term><term>Cdna</term><term>Cdna synthesis</term><term>Cetus corp</term><term>Consensus sequence</term><term>Core gene</term><term>Core particles</term><term>Duck hepatitis</term><term>Final volume</term><term>Fusion protein</term><term>Gene product</term><term>Genetic analysis</term><term>Genome</term><term>Ground squirrel hepatitis virus</term><term>Hantz</term><term>Hepatitis</term><term>Human hepatitis</term><term>Human liver tissues</term><term>Hybridization</term><term>Important role</term><term>Initiation codon</term><term>Lesser extent</term><term>Life cycle</term><term>Liver samples</term><term>Negative control</term><term>Noninfected woodchuck</term><term>Northern blot analysis</term><term>Nucleotide sequence</term><term>Oligonucleotide</term><term>Oligonucleotide probe</term><term>Oligonucleotide probes</term><term>Open reading frames</term><term>Poly</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Positive control</term><term>Potential translation product</term><term>Pregenomic</term><term>Primer</term><term>Primer pair</term><term>Probe</term><term>Rna</term><term>Schaller</term><term>Sequence analysis</term><term>Southern blot analysis</term><term>Spacer region</term><term>Splice</term><term>Splice donor site</term><term>Splice junction</term><term>Stringent conditions</term><term>Surface antigen</term><term>Suzuki</term><term>Terminal protein</term><term>Terminal protein domain</term><term>Transcript</term><term>Transcriptase</term><term>Transcription</term><term>Transcription step</term><term>Viral</term><term>Viral particles</term><term>Woodchuck</term><term>Woodchuck hepatitis virus</term><term>Woodchuck liver</term><term>Woodchuck liver tissues</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and partially sequenced. The splicing donor and acceptor sites of this novel RNA are located, respectively, 130 nucleotides downstream of the ATG initiation codon of the core gene and 21 nucleotides upstream of the initiation codon of the pre-S2 surface gene. The splicing event generates a new core-polymerase fusion protein and removes the terminal protein domain and the spacer region of the polymerase gene. A nucleotide probe specific for the splice junction was used following RT-PCR, to further confirm the existence of this spliced RNA in the liver of seven WHV-infected woodchucks. Deleted viral DNA molecules corresponding to the 2.4 kb spliced RNA were also detected in the liver and, to a lesser extent, in the serum of infected woodchucks, suggesting that this spliced RNA can be encapsidated and reverse-transcribed during the course of natural WHV infection.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><country name="France"><noRegion><name sortKey="Hantz, Olivier" sort="Hantz, Olivier" uniqKey="Hantz O" first="Olivier" last="Hantz">Olivier Hantz</name></noRegion><name sortKey="Baginski, Isabelle" sort="Baginski, Isabelle" uniqKey="Baginski I" first="Isabelle" last="Baginski">Isabelle Baginski</name><name sortKey="Chemin, Isabelle" sort="Chemin, Isabelle" uniqKey="Chemin I" first="Isabelle" last="Chemin">Isabelle Chemin</name><name sortKey="Fourel, Isabelle" sort="Fourel, Isabelle" uniqKey="Fourel I" first="Isabelle" last="Fourel">Isabelle Fourel</name><name sortKey="Trepo, Christian" sort="Trepo, Christian" uniqKey="Trepo C" first="Christian" last="Trepo">Christian Trepo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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